Genomics Laboratory

DNA and RNA analysis

  • human and animal (blood, serum, plasma, tissue homogenates, body fluids, etc.)
  • vegetable
  • cell cultures

Provided services include:

DNA analysis:
  • detection of rare mutations
  • detection of rare sequences
  • analysis of free, circulating DNA from tumor cells (ctDNA)
  • single nucleotide polymorphism (TaqMan SNP Genotyping Assays)
  • telomere length
  • analysis of cfDNA quantity and integrity in body fluids
  • sequencing of bacterial plasmids
  • sequencing of amplicons
  • analysis of DNA fragments
  • analysis of DNA methylation profile in cells
  • MLPA (Multiplex Ligation-dependent Probe Amplification) analysis
  • species identification of organisms
  • implementation of projects according to the ideas and needs of the client
RNA analysis:
  • gene expression (optimized kits for single gene expression testing or custom configured 96-well plates and small arrays for simultaneous analysis of multiple gene expression – from 8 to 380)
  • miRNA expression (kits for single miRNA quantifiation and small density arrays for the analysis of most known human, murine and rat miRNAs)
  • detection and quantification of exosomal miRNAs
  • implementation of projects according to the client requirements

Methods:

  • polymerase chain reaction – PCR
  • real-time PCR
  • reverse transcription
  • droplet digital PCR – ddPCR
  • sequencing

Available equipment:

  • Droplet Digital PCR (ddPCR; BioRad)
  • 7900 HT Fast Real-Time PCR System (Applied Biosystems)
  • Genetic Analyzer/16 Capillary Sequencer (Hitachi/Applied Biosystems)
  • MCE-202 MultiNA (Shimadzu)
  • Rotor-Gene™ 3000 – Real-Time PCR System (Corbett Research)

For service details please contact: